Ca-Alginate-PEGMA Hydrogels for In Situ Delivery of TGF-β Neutralizing Antibodies in a Mouse Model of Wound Healing

Hydrogels provide effective alternatives for drug delivery when therapeutics cannot be applied directly to a wound, or if adverse effects are associated with systemic administration. However, vehicles need biocompatible and biodegradable exhibit sufficient mechanical strength withstand handling different physiological conditions, such as those encountered during topical administration of therapeutic. Wound healing can divided into three phases stimulated by transforming growth factor-beta (TGF-β) and, subsequently, targeted have been developed inhibit this cytokine the treatment chronic wounds prevent scarring. In study, capacity calcium alginate hydrogels plasticized poly(ethylene glycol) methyl ether methacrylate (PEGMA) deliver anti-TGF-β antibodies (1D11.16.8) wound was investigated in situ. Three levels antibodies, 10, 50, 100 μg, were loaded calcium-alginate-PEGMA evaluated an excisional model mice. containing 50 μg 1D11.16.8 produced less inflammation, accompanied marked reduction collagen deposition cell infiltration. These findings demonstrate larger proteins, site wound.